ABSTRACT
The dried latex of Calotropis gigantean L. was tested for its analgesic, antioxidant and cytotoxic activity in mice. The latex significantly and dose dependently inhibited acetic acid induced writhing by 79.13% and 39.4% at 500 and 250 mg/kg body weight, respectively, comparable to diclofenac sodium. The antioxidant activity was determined qualitatively based on the scavenging activity of 1,1-dipheny 1-2-picryl hydrazyl [DPPH] free radical showing the presence of antioxidant principle. In brine shrimp lethality assay the latex was toxic with LC[50] and LC[90] values of 1.7 micro g/ml and 10 micro g/ ml, respectively. These results suggest that latex possess analgesic and antioxidant activities supporting its traditional uses. However, C. gigantea latex should be used with caution as it also showed toxicity, but more experiments are required in its support
ABSTRACT
The leaves of Anisomeles indica was tested for its antioxidant, analgesic, and antidiarrhoeal activities in experimental animals. The extract showed the presence of antioxidant principle qualitatively based on the scavenging activity of 1,1-diphenyl-2-picryl hydrazyl [DPPH] free radical. It showed the presence of antioxidant principle. The extract dose dependently inhibited the acetic acid induced writhing in mice [63.05%, P<0.001 and 37.63% for 500 and 250 mg/kg body weight respectively], the effect was significant comparable to the standard drug Diclofenac Sodium [54.92%, P<0.001] at the dose of 25 mg/kg. The extract of A. indica offered about 1.20 hrs and 3.7 hrs [P<0.001] of the mean latent period for diarrhoeal episode at the doses of 250 and 500 mg/kg body weight which is comparable to standard drug Loperamide. The results tend to suggest that the leaves might possess analgesic, antioxidant and antidiarrhoeal activities or active constituent[s] responsible for the activities and supported its traditional uses
Subject(s)
Animals, Laboratory , Plant Extracts , Plant Leaves , Antioxidants , Analgesics , Antidiarrheals , MiceABSTRACT
The aim of the present study is to investigate the antioxidant, neuropharmacological, analgesic and antimicrobial activities of the EtOH extract of the whole plant of Leucas zeylanica. Phytochemical screening indicated the presence of gums, carbohydrate, reducing sugar, phenols, tannins [gallic acid], cardiac glycosides and saponins. TLC-based antioxidant assay by DPPH free radical revealed positive activity of the extract and in quantitative assay IC[50] value was 62.9 microg/ml. The extract potentiated the phenobarbitone-induced sleeping time in mice in a dose dependent manner. From the study of analgesic activity using the model of acetic acid-induced writhing in mice it was found that the extract exhibited [***p<0.001] writhing inhibition was dose dependent. The extract showed moderate antimicrobial activity